There was a dose dependent difference in the speed of action of each antibiotic tested at supra-MIC concentrations. Quantitative measurement of the different nutrient effects on bacteria were obtained in less than four hours and it was possible to distinguish differences in the growth dynamics of Klebsiella pneumoniae 1705 possessing the Bla KPC betalactamase vs. We established the limit of detection for SLIC to be between 10 and 100 cfu mL −1 in a volume of 1–2 mL. Bacteria were also exposed meropenem, gentamicin and cefoxitin at a range of concentrations and light scattering of the liquid culture was captured in real-time. Scattering data was modelled using Gompertz and Broken Stick equations. We compared the light-scattering of bacteria grown in varying high and low nutrient liquid medium and the growth dynamics of two closely related organisms. We developed and tested a scattered light and detection system (SLIC) to address this challenge, establishing the limit of detection, and time to positive detection of the growth of small inocula. This means studies of antibiotic resistance and other physiological methods often take 24 h or longer. Understanding the response of bacteria to environmental stress is hampered by the relative insensitivity of methods to detect growth.
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